Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification

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Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification.

The action of non-detergent sulphobetaines (NDSBs) as new mild agents for protein purification is described. The solubilization effects of non-detergent sulphobetaines are shown in different examples; all obtained under non-denaturing conditions: (1) microsomal proteins extraction; (2) recovery after dialysis of nuclear proteins; (3) reduction of precipitation in isoelectric focusing experiment...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1995

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj3050337